A Laboratory Guide to Glycoconjugate Analysis, Vol. 9 ANNOTATION
The book contains black-and-white illustrations.
FROM THE CRITICS
Eugene Thonar
This book offers a broad selection of current methods for the detection, isolation, analysis, and biomolecular interactions of the carbohydrate moieties of glycoconjugates. It emphasizes methodology and focuses on methods of analysis that can be established in most research laboratories. The purpose is to provide the techniques as fully as possible but in a user-friendly way. This gives the reader all the necessary technical information required to implement techniques with minimal difficulty. Leaders from the fields of industry and academia do an outstanding job of reaching this worthwhile goal. Presenting protocols that can be easily followed by experienced researchers and aspiring novices alike, the expert contributors go out of their way to outline possible problems, identifying difficulties that may arise and offering tips that should prove invaluable in optimizing the experimental conditions and in yielding the best results. Each chapter begins with a presentation of the rationale for using a particular method and includes a detailed description of what materials/equipment are needed, how to develop and refine the technique in the laboratory, and how to interpret the results. Illustrations and references are used judiciously so as not to deluge the reader with information not directly relevant to the development of the methodology. The editors have done an outstanding job of ensuring uniformity of presentation throughout the book. This remarkable compilation of state-of-the-art techniques for the analysis of carbohydrate moieties is a must for any laboratory even peripherally involved in the study of glycoproteins, proteoglycans, and other glycoconjugates.
Doody Review Services
Reviewer: Eugene Thonar, PhD (Rush Medical College of Rush University)Description: This book offers a broad selection of current methods for the detection, isolation, analysis, and biomolecular interactions of the carbohydrate moieties of glycoconjugates. It emphasizes methodology and focuses on methods of analysis that can be established in most research laboratories. Purpose: The purpose is to provide the techniques as fully as possible but in a user-friendly way. This gives the reader all the necessary technical information required to implement techniques with minimal difficulty. Leaders from the fields of industry and academia do an outstanding job of reaching this worthwhile goal. Audience: Presenting protocols that can be easily followed by experienced researchers and aspiring novices alike, the expert contributors go out of their way to outline possible problems, identifying difficulties that may arise and offering tips that should prove invaluable in optimizing the experimental conditions and in yielding the best results. Features: Each chapter begins with a presentation of the rationale for using a particular method and includes a detailed description of what materials/equipment are needed, how to develop and refine the technique in the laboratory, and how to interpret the results. Illustrations and references are used judiciously so as not to deluge the reader with information not directly relevant to the development of the methodology. Assessment: The editors have done an outstanding job of ensuring uniformity of presentation throughout the book. This remarkable compilation of state-of-the-art techniques for the analysis of carbohydrate moieties is a must for any laboratory even peripherally involved in the study of glycoproteins, proteoglycans, and other glycoconjugates.
Booknews
A reference on methodology for both academic and industrial
laboratories. Details proven and effective methods for analyzing
complex carbohydrates that occur in biological systems, including new
methods for analyzing molecular interactions of glycans and
glycosaminoglycans. The operations considered include oligosaccharide
analysis, profiling, sequencing using enzymes, and glycan
identification and derivatization. Presented in sections such as a
comparison of chemical and enzymatic scission for determining the
disaccharide composition of glycosaminoglycans, the mass
spectrometric analysis of highly acidic polysaccharides, and the
high-performance liquid chromatography of derivatized and
non-derivatized oligosaccharides. The protocols are highlighted in
shaded boxes for quick reference.
Annotation c. by Book News, Inc., Portland, Or.
RATING
5 Stars! from Doody
ACCREDITATION
Jackson, Peter (Univ of Cambridge); Gallagher, John T. (Univ of Manchester)
The contributors represent the specialties of chemistry, biochemistry, medical oncology, and pharmaceutical science. Most come from universities and institutes in the U.S. and the U.K. Institutions prominently represented include MIT, Harvard, Glycobiology Institute, and Univ of Oxford.
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