A-Z of Quantitative PCR FROM THE CRITICS
Book News
Bustin (School of Medicine and Dentistry, University of London, UK) and contributors explain features of quantitative PCR (qPCR) and provide alternative viewpoints and methods in this hands-on manual for novice and expert researchers who want to learn how to set up and carry out qPCR assays from scratch. Early chapters give insight on factors that influence a PCR experiment and principles underlying real-time quantification. The core of the book details the steps involved in conducting a qPCR experiment, from sample collection to interpretation of results. Final chapters provide a protocol for mRNA quantification and guidelines for standardization of qPCR assays.
David G. Ginzinger - PhD
Laboratory Director, Genome Analysis Core Facility
UCSF Comprehensive Cancer Center
Dr. Stephen Bustin has done a remarkable job on the aptly titled A-Z of Quantitative PCR. It has remarkable depth to satisfy the needs of the most experienced Q-PCR scientist as well as the breadth to inspire the novice. Very clearly written and easy to use protocols and helpful tips ensure success when attempting your first real-time Q-PCR experiment. An amazingly rich resource for everything you ever wanted to know about real-time quantitative PCR-from the historical background, to chemistries, instruments, and data analysis. This book will quickly become an essential reference manual for every lab wanting to perform quantitative PCR. If after reading this book and following the recommendations you are not able to get Q-PCR to work, you should seriously consider changing careers.
Gregory L. Shipley - Ph.D.,
Director,
Quantitative Genomics Core Laboratory,
Department of Integrative Biology and Pharmacology-
The University of Texas Health Science Center- Houston
The book "A-Z of Quantitative PCR" edited by Dr. Stephen Bustin is, for once, exactly what the title claims, everything you want and need to know about real-time quantitative PCR. Since its' inception in 1996, real-time quantitative PCR has grown dramatically in the assortment of available hardware, chemistries and uses for this extremely robust technology. However, there has been no single reference to point new users to that completely covered all facets of this technology. The A-Z of Quantitative PCR fills that void. The material is presented in a very readable form for those new to the technique and yet has plenty of detailed information for experienced veterans. The book is divided into three sections beginning with threee excellent overviews of the technique followed by several chapters covering the basic principles behind every aspect of preparing samples to performing the RT and PCRs to how the various assay chemistries and available instrumentation work. Finally, there are quite a few chapters devoted to specific applications of the technology. A real plus are the appendices covering many handy biochemical facts and a glossary of terms for the newly initiated. All in all a very readable and informative book that should be a must read for anyone wanting to get into the field of real-time quantitative PCR.
WHAT PEOPLE ARE SAYING
Dr. Stephen Bustin has done a remarkable job on the aptly titled A-Z of Quantitative PCR. It has remarkable depth to satisfy the needs of the most experienced Q-PCR scientist as well as the breadth to inspire the novice. Very clearly written and easy to use protocols and helpful tips ensure success when attempting your first real-time Q-PCR experiment. An amazingly rich resource for everything you ever wanted to know about real-time quantitative PCR-from the historical background, to chemistries, instruments, and data analysis. This book will quickly become an essential reference manual for every lab wanting to perform quantitative PCR. If after reading this book and following the recommendations you are not able to get Q-PCR to work, you should seriously consider changing careers. — Laboratory Director, Genome Analysis Core Facility
UCSF Comprehensive Cancer Center
David G. Ginzinger
Dr. Stephen Bustin has done a remarkable job on the aptly titled A-Z of Quantitative PCR. It has remarkable depth to satisfy the needs of the most experienced Q-PCR scientist as well as the breadth to inspire the novice. Very clearly written and easy to use protocols and helpful tips ensure success when attempting your first real-time Q-PCR experiment. An amazingly rich resource for everything you ever wanted to know about real-time quantitative PCR-from the historical background, to chemistries, instruments, and data analysis. This book will quickly become an essential reference manual for every lab wanting to perform quantitative PCR. If after reading this book and following the recommendations you are not able to get Q-PCR to work, you should seriously consider changing careers. — Laboratory Director, Genome Analysis Core Facility
UCSF Comprehensive Cancer Center
Gregory L. Shipley
The book "A-Z of Quantitative PCR" edited by Dr. Stephen Bustin is, for once, exactly what the title claims, everything you want and need to know about real-time quantitative PCR. Since its' inception in 1996, real-time quantitative PCR has grown dramatically in the assortment of available hardware, chemistries and uses for this extremely robust technology. However, there has been no single reference to point new users to that completely covered all facets of this technology. The A-Z of Quantitative PCR fills that void. The material is presented in a very readable form for those new to the technique and yet has plenty of detailed information for experienced veterans. The book is divided into three sections beginning with threee excellent overviews of the technique followed by several chapters covering the basic principles behind every aspect of preparing samples to performing the RT and PCRs to how the various assay chemistries and available instrumentation work. Finally, there are quite a few chapters devoted to specific applications of the technology. A real plus are the appendices covering many handy biochemical facts and a glossary of terms for the newly initiated. All in all a very readable and informative book that should be a must read for anyone wanting to get into the field of real-time quantitative PCR. — Ph.D.
Director
Quantitative Genomics Core Laboratory
Department of Integrative Biology and Pharmacology
The University of Texas Health Science Center- Houston
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