Culture of Animal Cells: A Manual of Basic Technique ANNOTATION
The book contains predominantly black-and-white illustrations, with some color illustrations.
This book is a training manual for the fundamental principles and techniques of animal cell culture. Cell culture refers to cultures derived from dispersed cells taken from the original tissue, from a primary culture, or from a cell line or cell strain by enzymatic, mechanical, or chemical disaggregation. This edition includes major updates in the areas of biology, media, specialized techniques, biotechnology, and tumor culture, as well as references and suppliers.
FROM THE PUBLISHER
Provides an accessible, comprehensive synthesis of proven techniques for the culture and experimental manipulation of animal cells. Contains step-by-step laboratory procedures, detailed instructions for reagent preparation and information on the latest specialized equipment and supplies. This edition includes over 350 new references, 13 new protocols plus recent methods and expanded protocols in areas of new technology such as antibody staining and cytotoxicity assay.
SYNOPSIS
The fourth edition of Culture of Animal Cells: A Manual of Basic Technique offers the most complete training manual of its kind on the fundamental principles and techniques of animal cell culture. Within this volume, indispensable updates reflecting the latest progress in media, specialized techniques, biotechnology, DNA transfer, and tumor culture have been made. This edition has five new chapters expanding on serum-free media, scale-up and biofermentors, molecular techniques, immortalization, and troubleshooting.
The advantages of tissue culture go beyond control of the physiochemical environment and physiological conditions as shown in the comprehensive coverage of tissue culture topics, both organ culture and cell culture, provided in this manual. A wide range of essential information from basic to specialized procedures is presented, highlighting advantages and limitations, and illustrating the properties of different types of culture.
This crucial reference for cell culture techniques includes:
New Atlas of Cells section in full-color presentation Extended coverage of molecular techniques, scale-up, and serum-free medium New chapter on problem solving Photographs of cell lines, contaminations, and equipment Clear and concise tables and charts Educated recommendations on safety issues, ethical consent, and ownership
Biomedical researchers in cell biology, cytology, molecular biology, immunology, neuroscience, toxicology, and cancer biology will find Culture of Animal Cells: A Manual of Basic Technique, Fourth Edition to be an invaluable reference.
FROM THE CRITICS
SciTech Book News
...Freshney provides 28 chapters that reflect the latest progress in media, specialized techniques, biotechnology, DNA transfer and tumor culture.
SciTech Book News
...Freshney provides 28 chapters that reflect the latest progress in media, specialized techniques, biotechnology, DNA transfer and tumor culture.
Alvin Telser
This is a book on the techniques, equipment, practices, and principles needed to place animal cells into tissue culture. This volume is in its fourth edition. The book is intended to provide a complete technical guide to the growth of animal cells in tissue culture. Like other high quality techniques books, these are worthy objectives and are met admirably. According to the author, this book is written for persons with no previous experience with these techniques. It is extremely well written for such an audience. The author is a very well recognized expert in the field. The full range of information pertinent to the growth of animal cells in tissue culture is covered such as lab layout, necessary equipment, water quality, selection of media and growth chambers, steps starting with whole animal material, observing and staining cultured cells, common contaminants, and more. It is well illustrated. The black-and-white diagrams are very well done and helpful; many of the black-and-white photographs are also very good, but some are of moderate quality, especially many photomicrographs of cells. The newly included center section of color photos is likely to be of marginal value--partly due to low quality original material and partly due to poor quality reproduction. This will be a very helpful reference in any lab actively using tissue culture. It would also be a very useful reference volume in any life science library. The techniques used in tissue culture, like most fields in modern biology, changes fairly frequently, making a new edition every few years important. This book is well worth its cost.
Doody Review Services
Reviewer: Alvin Telser, PhD (Northwestern University Medical School)Description: This is a book on the techniques, equipment, practices, and principles needed to place animal cells into tissue culture. This volume is in its fourth edition. Purpose: The book is intended to provide a complete technical guide to the growth of animal cells in tissue culture. Like other high quality techniques books, these are worthy objectives and are met admirably. Audience: According to the author, this book is written for persons with no previous experience with these techniques. It is extremely well written for such an audience. The author is a very well recognized expert in the field. Features: The full range of information pertinent to the growth of animal cells in tissue culture is covered such as lab layout, necessary equipment, water quality, selection of media and growth chambers, steps starting with whole animal material, observing and staining cultured cells, common contaminants, and more. It is well illustrated. The black-and-white diagrams are very well done and helpful; many of the black-and-white photographs are also very good, but some are of moderate quality, especially many photomicrographs of cells. The newly included center section of color photos is likely to be of marginal value:partly due to low quality original material and partly due to poor quality reproduction. Assessment: This will be a very helpful reference in any lab actively using tissue culture. It would also be a very useful reference volume in any life science library. The techniques used in tissue culture, like most fields in modern biology, changes fairly frequently, making a new edition every few years important. This book is well worth its cost.
Booknews
New edition of a training manual on the fundamental principles and techniques of animal cell culture. Freshney (medical oncology, CRC Beatson Laboratories) provides 28 chapters that reflect the latest progress in media, specialized techniques, biotechnology, DNA transfer, and tumor culture. New chapters expand on serum-free media, scale-up and biofermentors, molecular techniques, immortalization, and troubleshooting. A wide range of essential information from basic to specialized procedures highlights the advantages and limitations of each one, and discusses the properties of different types of culture. Illustrations include a color atlas section; photographs of cell lines, contaminations, and equipment; and tables and charts. Annotation c. Book News, Inc., Portland, OR (booknews.com)
RATING
4 Stars! from Doody
ACCREDITATION
Freshney, R. Ian (Univ of Glasgow)
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